We injected NgAgo mRNA mixed with two complementary gDNAs targeting fabp11a and found that the injection caused abnormal eye phenotype but failed to introduce indels

In summary, our study shows that gene knockdown is the main mechanism by which gDNA/NgAgo affects gene function in zebrafish. This is supported by results of the experiments using presumably catalytically dead NgAgo. In addition, we have failed to detect any mutation in all the embryos we examined.